Objective: The aim of this study to present a full spectrum of individual presentations of pancreatic fistula risk patients, and to determine the utility of mitigation strategies among some of the most common and vulnerable scenario surgeon meeting.
Background: FRS has been used to identify technical strategies related to reducing the incidence of CR-POPF in various strata of risk. However, risk-stratification using the FRS not been investigated with greater granularity. By lowering all possible combinations of elements FRS, individual risk assessment can be used for medicinal purposes precision.
Methods: FRS profile and the results of the 5533 PD accrued from 17 international organizations (2003-2019). FRS is used to get 80 patients a unique combination of a “scenario.” risk-matched analysis was performed using a Bonferroni adjustment to identify the scenario with increased susceptibility to CR-POPF occurrence. Furthermore, these scenarios were analyzed using multivariable regression to explore the optimal mitigation approach.
Results: The overall rate of CR-POPF was 13.6%. All 80 scenarios that may be encountered, with the most frequent being the scenario # 1 (8.1%) – only negligible-risk scenario (level CR-POPF = 0.7%). Moderate-risk zone has the most scenario (50), patients (N = 3246), CR-POPFs (65.2%), and the largest non-zero-POPF CR rate differences between the scenarios (18-fold). In a risk-matched analysis, scenario 2 (# 59 and 60) displayed increased susceptibility to CR-POPF relative to moderate-risk zones (both P <0.001). Multivariate analysis revealed that factors associated with CR-POPF in this scenario: pancreaticogastrostomy reconstruction [odds ratio (OR) 4.67], negligence drain placement (OR 5.51), and octreotide prophylaxis (OR 3.09).
When comparing the utilization of best practice strategies for patients who do not have these jointly exploited, there is a significant reduction in CR-POPF (10.7% vs 35.5%, P <0.001; OR 0.20, 95% confidence interval 0, 12-.33). Conclusion: Through this data, the risk of fistula comprehensive catalog has been created and the most clinical scenario-impact has been seen. Focusing on individual scenario provides a practical way for the treatment of precision approach, allowing for more focused management and efficient CR-POPF.
The Fistula Risk Score Catalog: Toward Precision Medicine for Pancreatic Fistula After Pancreatoduodenectomy
The Gene Catalog Gut Microbiome and Comparative Analysis of Big Cats Gives New Insights in Panthera Species
Most of metagenomic research in the past decade have focused on expressing the human gut microbiomes, rodents and ruminants; However, the gut microbiome and genic information (catalog of genes) of felids such large Panthera species are largely unknown until now. In this study, the intestinal bacterial, fungal, and viral metagenomic rated composition of three species of Panthera (lions, leopards, and tigers) from India, which consume the same diet and belong to the same geographical location.
A catalog of non-redundant bacterial genes of Panthera intestine consists of 1,507,035 putative gene constructed of 27 individual Panthera, which revealed a higher abundance of purine metabolism genes associated with their dietary intake of purine-rich. Analysis of Carbohydrate Active enzyme (CAZy) and database enrichment Merops identified glycoside hydrolase (GHs), glycosides-transferase, and collagenase in the intestines, which is essential for the absorption of nutrients from animal biomass. Bacteria, fungi, and viruses community analysis provides first comprehensive insight into specific Panthera microbial communities.
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TET3. Recognizes TET3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Biolipidure does not require biohazardous handling.
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Puromycin marker under Rsv promoter.
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Puromycin marker under Rsv promoter.
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TET3. Recognizes TET3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TET3. Recognizes TET3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TET3. Recognizes TET3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Blasticidin fusion marker under Rsv promoter.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Puromycin fusion marker under Rsv promoter.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-Blasticidin fusion marker under Rsv promoter.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-puromycin fusion marker under Rsv promoter.
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Blasticidin fusion marker under Rsv promoter.
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Puromycin fusion marker under Rsv promoter.
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-Blasticidin fusion marker under Rsv promoter.
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-puromycin fusion marker under Rsv promoter.
Mouse IgA, IgGs (1, 2a, 2b, 3), IgM, and IgE isotype controls (set of 7 IgGs)
Description: Pre-made over-expression lentivirus for expressing human target: TET3 (tet methylcytosine dioxygenase 3), [alternative names: hCG_40738]. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_001287491.1 . It also contains a RFP-Blasticidin dual selection marker.
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Blasticidin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Puromycin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-Blasticidin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-puromycin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.
ELISA Microplate Washer Automated (#MPW-30) bottle set (3 bottles and cap assembly)
Panthera gene catalog and the largest comparative study of the composition of the gut bacteria of 68 individuals from carnivorous species from different geographic locations and diet underscores the role of diet and geography in shaping Panthera gut microbiome, which is significant for the health and conservation management of endangered species.
Human TET3 shRNA Plasmid 
Mouse TET3 shRNA Plasmid 
pROKII- RNAI Plasmid )
Tet3 sgRNA CRISPR Lentivector set (Mouse) )
TET3 sgRNA CRISPR Lentivector set (Human)  Antibody)
Methylcytosine Dioxygenase TET3 (TET3) Antibody 
GeneGlide? RNAi Delivery Control 
T7 RNAi Transcription Kit  Antibody (HRP))
Methylcytosine Dioxygenase TET3 (TET3) Antibody (HRP)  Antibody (FITC))
Methylcytosine Dioxygenase TET3 (TET3) Antibody (FITC)  Antibody (Biotin))
Methylcytosine Dioxygenase TET3 (TET3) Antibody (Biotin) 
TET3 Antibody 
anti-TET3 
Human Methylcytosine dioxygenase TET3, TET3 ELISA KIT 
Mouse Methylcytosine dioxygenase TET3, Tet3 ELISA KIT 
Set of 10 Biolipidure Reagents 
TET3 Rabbit pAb 
TET3 Polyclonal Antibody 
TET3 cloning plasmid 
Anti-TET3 antibody 
pCMV-SPORT6-TET3 , Sterile-Filtered)
StemBoost? YPAC Cocktail Set (1000X), Sterile-Filtered )
Tet3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) )
TET3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) , Sterile-Filtered)
StemBoost? Reprogramming Cocktail Set I (1000X), Sterile-Filtered , Sterile-Filtered)
StemBoost? Reprogramming Cocktail Set II (1000X), Sterile-Filtered , Sterile-Filtered)
StemBoost? 2i-Reprogramming Cocktail Set (1000X), Sterile-Filtered 
Antigen-Antibody Pens, a set of any 3 pens , Sterile-Filtered)
StemBoost? SMAD Signaling Inhibitor Cocktail Set (1000X), Sterile-Filtered , Sterile-Filtered)
StemBoost? Neuronal Cell Induction Cocktail Set (100X), Sterile-Filtered 
pAd/BLOCK-iT-DEST RNAi Gateway Vector -(blasticidin) lentivirus)
shRNA-H1 (Neg)-(blasticidin) lentivirus -(Puromycin) lentivirus)
shRNA-H1 (Neg)-(Puromycin) lentivirus -(blasticidin) lentivirus)
shRNA-U6 (Neg)-(blasticidin) lentivirus -(Puromycin) lentivirus)
shRNA-U6 (Neg)-(Puromycin) lentivirus 
TET3 Antibody, HRP conjugated 
TET3 Antibody, FITC conjugated 
TET3 Antibody, Biotin conjugated 
TET3 Polyclonal Conjugated Antibody  (Target 1))
Tet3 sgRNA CRISPR Lentivector (Mouse) (Target 1)  (Target 2))
Tet3 sgRNA CRISPR Lentivector (Mouse) (Target 2)  (Target 3))
Tet3 sgRNA CRISPR Lentivector (Mouse) (Target 3)  (Target 1))
TET3 sgRNA CRISPR Lentivector (Human) (Target 1)  (Target 2))
TET3 sgRNA CRISPR Lentivector (Human) (Target 2)  (Target 3))
TET3 sgRNA CRISPR Lentivector (Human) (Target 3) -( GFP-Bsd) lentivirus)
shRNA-H1 (Neg)-( GFP-Bsd) lentivirus -( GFP-Puro) lentivirus)
shRNA-H1 (Neg)-( GFP-Puro) lentivirus -( RFP-Bsd) lentivirus)
shRNA-H1 (Neg)-( RFP-Bsd) lentivirus -( RFP-Puro) lentivirus)
shRNA-H1(Neg)-( RFP-Puro) lentivirus -( GFP-Bsd) lentivirus)
shRNA-U6 (Neg)-( GFP-Bsd) lentivirus -( GFP-Puro) lentivirus)
shRNA-U6 (Neg)-( GFP-Puro) lentivirus -( RFP-Bsd) lentivirus)
shRNA-U6 (Neg)-( RFP-Bsd) lentivirus -( RFP-Puro) lentivirus)
shRNA-U6 (Neg)-( RFP-Puro) lentivirus , IgM, and IgE isotype controls (set of 7 IgGs))
Mouse IgA, IgGs (1, 2a, 2b, 3), IgM, and IgE isotype controls (set of 7 IgGs) 
TET3 Polyclonal Antibody, HRP Conjugated 
TET3 Polyclonal Antibody, FITC Conjugated 
TET3 Polyclonal Antibody, Biotin Conjugated  (pORF))
Tet3 ORF Vector (Mouse) (pORF)  (pORF))
TET3 ORF Vector (Human) (pORF) 
h TET3 inducible lentiviral particles 
pECMV-Tet3-m-FLAG Plasmid 
50ml TC Tubes, Conical, 440 units/box 
GMP IL4, 50µg 
rHu IL 2 , 3MIU 
rHu IL 2 , 3MIU , Lot 200908F02 
PRE-GMP rHu GM-CSF, Molgramostim-Leukoma 
SET siRNA -( GFP-Bsd) lentivirus in PBS)
shRNA-H1 (Neg)-( GFP-Bsd) lentivirus in PBS -( GFP-Puro) lentivirus in PBS)
shRNA-H1 (Neg)-( GFP-Puro) lentivirus in PBS -( RFP-Bsd) lentivirus in PBS)
shRNA-H1 (Neg)-( RFP-Bsd) lentivirus in PBS -( RFP-Puro) lentivirus in PBS)
shRNA-H1(Neg)-( RFP-Puro) lentivirus in PBS  bottle set (3 bottles and cap assembly))
ELISA Microplate Washer Automated (#MPW-30) bottle set (3 bottles and cap assembly)  (pPB-C-His))
TET3 Protein Vector (Human) (pPB-C-His)  (pPB-N-His))
TET3 Protein Vector (Human) (pPB-N-His)  (pPM-C-HA))
TET3 Protein Vector (Human) (pPM-C-HA)  (pPM-C-His))
TET3 Protein Vector (Human) (pPM-C-His) 
TET3 3'UTR Luciferase Stable Cell Line  (pPB-C-His))
TET3 Protein Vector (Mouse) (pPB-C-His)  (pPB-N-His))
TET3 Protein Vector (Mouse) (pPB-N-His)  (pPM-C-HA))
TET3 Protein Vector (Mouse) (pPM-C-HA)  (pPM-C-His))
TET3 Protein Vector (Mouse) (pPM-C-His) 
Tet3 3'UTR Luciferase Stable Cell Line 
TET3 3'UTR GFP Stable Cell Line 
Tet3 3'UTR GFP Stable Cell Line 
FURA-4F/AM 
FURA-5F/AM 
Triton® X-100, MegaPure? Detergent, 10% Solution 
Tween® 20, MegaPure? Detergent, 10% Solution 
Tween® 80, MegaPure? Detergent, 10% Solution